Publications

Research articles

ImageDescriptionPDF File
SPICERBrukman NG, Valansi C, Podbilewicz B. "Sperm induction of somatic cell-cell fusion as a novel functional test". Elife. 2024 Jan 24;13. doi: 10.7554/eLife.94228. PubMed PMID: 38265078. Link
VirusesHindi SM, Petrany MJ, Greenfeld E, Focke LC, Cramer AAW, Whitt MA, Khairallah RJ, Ward CW, Chamberlain JS, Prasad V, Podbilewicz B, Millay DP. Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery. Cell. 2023 Aug 3;186(16):3520. doi: 10.1016/j.cell.2023.06.025. PubMed PMID: 37541201. Link
IZUMO1 is a sperm fusogen.Brukman N.G., Nakajima K.P., Valansi C., Li X. Higashiyama T., Podbilewicz B. "A novel function for the sperm adhesion protein IZUMO1 in cell–cell fusion" J Cell Biol (2023) 222 (2): e202207147. Link
TModel for PfHAP2 and PfHAP2p function during Plasmodium gamete fusion.Kumar S., Valansi C., Haile M.T., Li X., Flyak K., Dwivedy A., Abatiyow B.A., Leeb A.S., Kennedy S.Y., Camargo N.M., Vaughan A.M., Brukman N.G., Podbilewicz B., Kappe SHI. (2022) "Malaria parasites utilize two essential plasma membrane fusogens for gamete fertilization" Cell Mol Life Sci. 79(11):549. Link
Tree of FsxAs, surface charge and environments.Moi D., Nishio S., Li X., Valansi C., Langleib M., Brukman N.G., Flyak K., Dessimoz C., de Sanctis D., Tunyasuvunakool K., Jumper J., Grana M., Romero H., Aguilar P.S., Jovine L., Podbilewicz B. (2022) "Discovery of archaeal fusexins homologous to eukaryotic HAP2/GCS1 gamete fusion proteins" Nat Commun 13, 3880 (2022). Link
Live imaging-based assay for visualising species-specific interactions in gamete adhesion molecules.Nakajima K.P., Valansi C., Kurihara D., Sasaki N., Podbilewicz B. &
Higashiyama T. (2022) "Live imaging-based assay for visualising species-specific interactions in gamete adhesion molecules" Sci Rep. 2022 Jun 10;12(1):9609.		PDF
Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegansYuval O., Iosilevskii Y., Meledin A., Podbilewicz B., Shemesh T. (2021) "Neuron tracing and quantitative analyses of dendritic architecture reveal symmetrical three-way-junctions and phenotypes of git-1 in C. elegans" PLoS Comput Biol 17(7): e1009185. Link
EFF-1 promotes muscle fusion, paralysis and retargets infection by AFF-1-coated viruses in C. elegansMeledin A., Li X., Matveev E., Gildor B., Katzir O. & Podbilewicz B. (2020). "EFF-1 promotes muscle fusion, paralysis and retargets infection by AFF-1-coated viruses in C. elegans".
bioRxiv (Preprint).		 Link
Sensory experience controls dendritic structure and behavior by distinct pathways involving degenerinsInberg, N., Iosilevskii Y. & Podbilewicz, B. (2018). "Sensory experience controls dendritic structure and behavior by distinct pathways involving degenerins".
bioRxiv 436758 (Preprint).
 Link
The fusogen AFF-1 can rejuvenate the regenerative potentialKravtsov, V., Oren-Suissa, M., & Podbilewicz, B. (2017). "AFF-1 fusogen can rejuvenate the regenerative potential of adult dendritic trees via self-fusion". Development, dev-150037.PDF
Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogensValansi, C., D. Moi, E. Leikina, E. Matveev, M. Graña, L. V. Chernomordik, H. Romero, P. S. Aguilar and B. Podbilewicz (2017). "Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens." The Journal of Cell Biology.PDF
Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion in Caenorhabditis elegans.Oren-Suissa, M., Gattegno, T., Kravtsov, V., Podbilewicz, B. (2017).Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion in Caenorhabditis elegans.
Genetics Early online March 10, 2017; DOI: 10.1534/genetics.116.196386		PDF
ksenia2016Smurova, K. and Podbilewicz, B. (2016) RAB-5-and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion. Cell reports, 14(6), pp.1517-1527.PDF
Dendritic tree extraction from noisy maximum intensity projection images in C. elegansGreenblum, A., Sznitman, R., Fua, P., Arratia, P.E., Oren, M., Podbilewicz, B., and Sznitman, J. (2014). Dendritic tree extraction from noisy maximum intensity projection images in C. elegans. Biomedical engineering online 13, 74.PDF
Structural basis of eukaryotic cell-cell fusionPerez-Vargas, J., Krey, T., Valansi, C., Avinoam, O., Haouz, A., Jamin, M., Raveh-Barak, H., Podbilewicz, B., and Rey, F.A. (2014). Structural basis of eukaryotic cell-cell fusion. Cell 157, 407-419.PDF
Axon regrowth during development and regeneration following injury share molecular mechanismsYaniv, S.P., Issman-Zecharya, N., Oren-Suissa, M., Podbilewicz, B., and Schuldiner, O. (2012). Axon regrowth during development and regeneration following injury share molecular mechanisms. Current biology : CB 22, 1774-1782.PDF
Conserved eukaryotic fusogens can fuse viral envelopes to cellsAvinoam, O., Fridman, K., Valansi, C., Abutbul, I., Zeev-Ben-Mordehai, T., Maurer, U.E., Sapir, A., Danino, D., Grunewald, K., White, J.M., et al. (2011). Conserved eukaryotic fusogens can fuse viral envelopes to cells. Science 332, 589-592PDF
The fusogen EFF-1 controls sculpting of mechanosensory dendritesOren-Suissa, M., Hall, D.H., Treinin, M., Shemer, G., and Podbilewicz, B. (2010). The fusogen EFF-1 controls sculpting of mechanosensory dendrites. Science 328, 1285-1288.PDF
Fusion-pore expansion during syncytium formation is restricted by an actin networkChen, A., Leikina, E., Melikov, K., Podbilewicz, B., Kozlov, M.M., and Chernomordik, L.V. (2008). Fusion-pore expansion during syncytium formation is restricted by an actin network. Journal of cell science 121, 3619-3628.PDF
Changing of the cell division axes drives vulva evolution in nematodesKolotuev, I., and Podbilewicz, B. (2008). Changing of the cell division axes drives vulva evolution in nematodes. Developmental biology 313, 142-154.PDF
Trends, stasis, and drift in the evolution of nematode vulva developmentKiontke, K., Barriere, A., Kolotuev, I., Podbilewicz, B., Sommer, R., Fitch, D.H., and Felix, M.A. (2007). Trends, stasis, and drift in the evolution of nematode vulva development. Current biology : CB 17, 1925-1937.PDF
Barrier to autointegration factor blocks premature cell fusion and maintains adult muscle integrity in C. elegansMargalit, A., Neufeld, E., Feinstein, N., Wilson, K.L., Podbilewicz, B., and Gruenbaum, Y. (2007). Barrier to autointegration factor blocks premature cell fusion and maintains adult muscle integrity in C. elegans. The Journal of cell biology 178, 661-673.PDF
AFF-1, a FOS-1-regulated fusogen, mediates fusion of the anchor cell in C. elegans.Sapir, A., Choi, J., Leikina, E., Avinoam, O., Valansi, C., Chernomordik, L.V., Newman, A.P., and Podbilewicz, B. (2007). AFF-1, a FOS-1-regulated fusogen, mediates fusion of the anchor cell in C. elegans. Developmental cell 12, 683-698.PDF
Genetic control of fusion pore expansion in the epidermis of Caenorhabditis elegansGattegno, T., Mittal, A., Valansi, C., Nguyen, K.C., Hall, D.H., Chernomordik, L.V., and Podbilewicz, B. (2007). Genetic control of fusion pore expansion in the epidermis of Caenorhabditis elegans. Molecular biology of the cell 18, 1153-1166.PDF
The C. elegans developmental fusogen EFF-1 mediates homotypic fusion in heterologous cells and in vivo. Podbilewicz, B., Leikina, E., Sapir, A., Valansi, C., Suissa, M., Shemer, G., and Chernomordik, L.V. (2006). The C. elegans developmental fusogen EFF-1 mediates homotypic fusion in heterologous cells and in vivo. Developmental cell 11, 471-481.PDF
ceh-16/engrailed patterns the embryonic epidermis of Caenorhabditis elegansCassata, G., Shemer, G., Morandi, P., Donhauser, R., Podbilewicz, B., and Baumeister, R. (2005). ceh-16/engrailed patterns the embryonic epidermis of Caenorhabditis elegans. Development 132, 739-749.PDF
EFF-1 is sufficient to initiate and execute tissue-specific cell fusion in C. elegansShemer, G., Suissa, M., Kolotuev, I., Nguyen, K.C., Hall, D.H., and Podbilewicz, B. (2004). EFF-1 is sufficient to initiate and execute tissue-specific cell fusion in C. elegans. Current biology : CB 14, 1587-1591.PDF
The small ubiquitin-like modifier (SUMO) is required for gonadal and uterine-vulval morphogenesis in Caenorhabditis elegansBroday, L., Kolotuev, I., Didier, C., Bhoumik, A., Gupta, B.P., Sternberg, P.W., Podbilewicz, B., and Ronai, Z. (2004a). The small ubiquitin-like modifier (SUMO) is required for gonadal and uterine-vulval morphogenesis in Caenorhabditis elegans. Genes & development 18, 2380-2391.PDF
The LIM domain protein UNC-95 is required for the assembly of muscle attachment structures and is regulated by the RING finger protein RNF-5 in C. elegans. Broday, L., Kolotuev, I., Didier, C., Bhoumik, A., Podbilewicz, B., and Ronai, Z. (2004b). The LIM domain protein UNC-95 is required for the assembly of muscle attachment structures and is regulated by the RING finger protein RNF-5 in C. elegans. The Journal of cell biology 165, 857-867.PDF
Pristionchus pacificus vulva formation: polarized division, cell migration, cell fusion, and evolution of invagination. Kolotuev, I., and Podbilewicz, B. (2004). Pristionchus pacificus vulva formation: polarized division, cell migration, cell fusion, and evolution of invagination. Developmental biology 266, 322-333.PDF
Control of vulval competence and centering in the nematode Oscheius sp. 1 CEW1Louvet-Vallee, S., Kolotuev, I., Podbilewicz, B., and Felix, M.A. (2003). Control of vulval competence and centering in the nematode Oscheius sp. 1 CEW1. Genetics 163, 133-146PDF
IN-39/Hox triggers cell division and represses EFF-1/fusogen-dependent vulval cell fusionShemer, G., and Podbilewicz, B. (2002). LIN-39/Hox triggers cell division and represses EFF-1/fusogen-dependent vulval cell fusion. Genes & development 16, 3136-3141.PDF
The type I membrane protein EFF-1 is essential for developmental cell fusionMohler, W.A., Shemer, G., del Campo, J.J., Valansi, C., Opoku-Serebuoh, E., Scranton, V., Assaf, N., White, J.G., and Podbilewicz, B. (2002). The type I membrane protein EFF-1 is essential for developmental cell fusion. Developmental cell 2, 355-362.PDF
Temperature-controlled microscopy for imaging living cells: apparatus, thermal analysis and temperature dependency of embryonic elongation in Caenorhabditis elegansRabin, Y., and Podbilewicz, B. (2000). Temperature-controlled microscopy for imaging living cells: apparatus, thermal analysis and temperature dependency of embryonic elongation in Caenorhabditis elegans. Journal of microscopy 199, 214-223.PDF
Ring formation drives invagination of the vulva in Caenorhabditis elegans: Ras, cell fusion, and cell migration determine structural fatesShemer, G., Kishore, R., and Podbilewicz, B. (2000). Ring formation drives invagination of the vulva in Caenorhabditis elegans: Ras, cell fusion, and cell migration determine structural fates. Developmental biology 221, 233-248.PDF
Formation of the vulva in Caenorhabditis elegans: a paradigm for organogenesisSharma-Kishore, R., White, J.G., Southgate, E., and Podbilewicz, B. (1999). Formation of the vulva in Caenorhabditis elegans: a paradigm for organogenesis. Development 126, 691-699.PDF
ADM-1, a protein with metalloprotease- and disintegrin-like domains, is expressed in syncytial organs, sperm, and sheath cells of sensory organs in Caenorhabditis elegans. Podbilewicz, B. (1996). ADM-1, a protein with metalloprotease- and disintegrin-like domains, is expressed in syncytial organs, sperm, and sheath cells of sensory organs in Caenorhabditis elegans. Molecular biology of the cell 7, 1877-1893.PDF
Cell fusions in the developing epithelial of C. elegansPodbilewicz, B., and White, J.G. (1994). Cell fusions in the developing epithelial of C. elegans. Developmental biology 161, 408-424.PDF

Review articles

ImageDescriptionPDF File
SchemeRomero H, Aguilar PS, Graña M, Langleib M, Gudiño V, Podbilewicz B. "Membrane fusion and fission during eukaryogenesis." Curr Opin Cell Biol. 2024 Jan 13;86:102321. Link
gamete fusionBrukman, N.G., X. Li and B. Podbilewicz. (2021). "Fusexins, HAP2/GCS1 and evolution of gamete fusion". Front. Cell Dev. Biol. 9:824024. Link
How cells fuseIosilevskii, Y. and Podbilewicz, B. (2021) "Programmed cell fusion in development and homeostasis" Curr Top Dev Biol. 2021;144:215-244. PDF
How cells fuseBrukman, N.G., B. Uygur, B. Podbilewicz, and L.V. Chernomordik. (2019). "How cells fuse". J Cell Biol. 218 (5): 1436-1451. PDF
Lessons from Worm Dendritic<br /> PatterningInberg, S., A. Meledin, V. Kravtsov, Y. Iosilevskii, M. Oren-Suissa, and B. Podbilewicz. (2019). "Lessons from worm dendritic patterning". Annual review of neuroscience. Vol. 42:365-383 PDF
The fusogen AFF-1 can rejuvenate the regenerative potentialSegev, N., Avinoam, O., & Podbilewicz, B. (2018). "Fusogens". Current Biology, 28(8), R378-R380. PDF
The hallmarks of cell-cell fusionHernandez, J.M. and Podbilewicz, B. (2017). The hallmarks of cell-cell fusion. Development.
144:4481-4495. 		PDF
Organogenesis of the C. elegans Vulva and Control of Cell FusionWeinstein, N., & Podbilewicz, B. (2016). Organogenesis of the C. elegans Vulva and Control of Cell Fusion. In Organogenetic Gene Networks (pp. 9-56). Springer International Publishing.PDF
Endocytosis regulates membrane localization and function of the fusogen EFF-1Smurova, K., & Podbilewicz, B. (2016). Endocytosis regulates membrane localization and function of the fusogen EFF-1. Small GTPasesPDF
Virus and cell fusion mechanismsPodbilewicz, B. (2014). Virus and cell fusion mechanisms. Annual review of cell and developmental biology 30, 111-139.PDF
Genetic basis of cell-cell fusion mechanismsAguilar, P.S., Baylies, M.K., Fleissner, A., Helming, L., Inoue, N., Podbilewicz, B., Wang, H., and Wong, M. (2013). Genetic basis of cell-cell fusion mechanisms. Trends in genetics : TIG 29, 427-437.PDF
Eukaryotic cell-cell fusion familiesAvinoam, O., and Podbilewicz, B. (2011). Eukaryotic cell-cell fusion families. Current topics in membranes 68, 209-234.PDF
Heterochronic control of AFF-1-mediated cell-to-cell fusion in C. elegans. Friedlander-Shani, L., and Podbilewicz, B. (2011). Heterochronic control of AFF-1-mediated cell-to-cell fusion in C. elegans. Advances in experimental medicine and biology 713, 5-11.PDF
Evolution of programmed cell fusion: common mechanisms and distinct functions. Oren-Suissa, M., and Podbilewicz, B. (2010). Evolution of programmed cell fusion: common mechanisms and distinct functions. Developmental dynamics : an official publication of the American Association of Anatomists 239, 1515-1528.PDF
Cell fusion in Caenorhabditis elegans. Alper, S., and Podbilewicz, B. (2008). Cell fusion in Caenorhabditis elegans. Methods in molecular biology 475, 53-74.
Viral and developmental cell fusion mechanisms: conservation and divergenceSapir, A., Avinoam, O., Podbilewicz, B., and Chernomordik, L.V. (2008). Viral and developmental cell fusion mechanisms: conservation and divergence. Developmental cell 14, 11-21.PDF
Cell fusion during developmentOren-Suissa, M., and Podbilewicz, B. (2007). Cell fusion during development. Trends in cell biology 17, 537-546.PDF
Cell fusionPodbilewicz, B. (2006). Cell fusion. WormBook : the online review of C elegans biology, 1-32.PDF
Cell fusion in development and disease. In Protein-Lipid Interactions: From Membrane Domains to Cellular Networks Podbilewicz, B., and Chernomordik, L.V. (2005). Cell fusion in development and disease. In Protein-Lipid Interactions: From Membrane Domains to Cellular Networks (Wiley-VCH Verlag GmbH Weinheim, Germany), pp. 175-207.PDF
Sweet control of cell migration, cytokinesis and organogenesis. Podbilewicz, B. (2004). Sweet control of cell migration, cytokinesis and organogenesis. Nature cell biology 6, 9-11.PDF
C. elegans. A Laboratory Manual. In Live Cell ImagingPodbilewicz, B., and Gruenbaum, Y.(2004). C. elegans. A Laboratory Manual. In Live Cell Imaging: Editors Spector, D.L. and Goldman, R.D.Cold Spring Harbor Laboratory Press.
How does a cell anchor and invade an organ? Podbilewicz, B. (2003). How does a cell anchor and invade an organ? Developmental cell 5, 5-7.PDF
The story of cell fusion: big lessons from little wormsShemer, G., and Podbilewicz, B. (2003). The story of cell fusion: big lessons from little worms. BioEssays : news and reviews in molecular, cellular and developmental biology 25, 672-682.PDF
cell fusion in organ formationShemer, G., and Podbilewicz, B. (2000). Fusomorphogenesis: cell fusion in organ formation. Developmental dynamics : an official publication of the American Association of Anatomists 218, 30-51.PDF
Membrane fusion as a morphogenetic force in nematode development. Podbilewicz, B. (2000). Membrane fusion as a morphogenetic force in nematode development. Nematology 2, 99-111.PDF

Featured publications

ImageDescriptionPDF file
IZUMO1 is a sperm fusogen.Brukman N.G., Nakajima K.P., Valansi C., Li X. Higashiyama T., Podbilewicz B. "A novel function for the sperm adhesion protein IZUMO1 in cell–cell fusion" J Cell Biol (2023) 222 (2): e202207147. Link
Tree of FsxAs, surface charge and environments.Moi D., Nishio S., Li X., Valansi C., Langleib M., Brukman N.G., Flyak K., Dessimoz C., de Sanctis D., Tunyasuvunakool K., Jumper J., Grana M., Romero H., Aguilar P.S., Jovine L., Podbilewicz B. (2022) "Discovery of archaeal fusexins homologous to eukaryotic HAP2/GCS1 gamete fusion proteins" Nat Commun 13, 3880 (2022). DOI: 10.1038/s41467-022-31564-1PDF
Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogensValansi, C., D. Moi, E. Leikina, E. Matveev, M. Graña, L. V. Chernomordik, H. Romero, P. S. Aguilar and B. Podbilewicz (2017). "Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens." The Journal of Cell Biology. DOI: 10.1083/jcb.201610093
PDF
Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion in Caenorhabditis elegans.Oren-Suissa, M., Gattegno, T., Kravtsov, V., Podbilewicz, B. (2017).Extrinsic Repair of Injured Dendrites as a Paradigm for Regeneration by Fusion in Caenorhabditis elegans.
Genetics Early online March 10, 2017; DOI: 10.1534/genetics.116.196386

PDF
Structural basis of eukaryotic cell-cell fusionPerez-Vargas, J., Krey, T., Valansi, C., Avinoam, O., Haouz, A., Jamin, M., Raveh-Barak, H., Podbilewicz, B., and Rey, F.A. (2014). Structural basis of eukaryotic cell-cell fusion. Cell 157, 407-419.PDF
Conserved eukaryotic fusogens can fuse viral envelopes to cellsAvinoam, O., Fridman, K., Valansi, C., Abutbul, I., Zeev-Ben-Mordehai, T., Maurer, U.E., Sapir, A., Danino, D., Grunewald, K., White, J.M., et al. (2011). Conserved eukaryotic fusogens can fuse viral envelopes to cells. Science 332, 589-592.PDF
The fusogen EFF-1 controls sculpting of mechanosensory dendritesOren-Suissa, M., Hall, D.H., Treinin, M., Shemer, G., and Podbilewicz, B. (2010). The fusogen EFF-1 controls sculpting of mechanosensory dendrites. Science 328, 1285-1288.PDF